英文名称DNAJC2

中文名称DNAJC2蛋白抗体

别    名Zuotin-1; AU020218; DnaJ (Hsp40) homolog subfamily C member 2; DnaJ homolog subfamily C member 2; Dnajc2; DNJC2_HUMAN; M phase phosphoprotein 11; M-phase phosphoprotein 11; MGC105894; MIDA1; MPHOSPH11; MPP11; OTTMUSP00000031226; ZRF1; Zrf2; ZUO1; Zuotin related factor 1; Zuotin related factor 2; Zuotin-related factor 1.   

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Rat,  (predicted: Mouse, Chicken, Cow, Horse, Rabbit, Sheep, )

产品应用WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量72kDa

细胞定位细胞核 细胞浆

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human DNAJC2: 521-621/621

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. [provided by RefSeq, Jul 2008]

如何选择抗体应用实验

 

首先根据自己要做的实验来筛选，有的抗体能应用很多实验，有的抗体则只能用于某一两个实验。

 

常见实验简写：IHC-P免疫组化石蜡切片、IHC-F免疫组化冰冻切片、mIHC多色荧光免疫组化、IF免疫荧光、ICC免疫细胞化学、WB(WesternBlot）蛋白质印迹试验、Flow Cyt (Intra)流式、IP免疫沉淀、ELISA酶联免疫吸附试验。

 

标记方式（偶联物）

 

偶联物从最终的图像采集来分可分为白光和荧光；

 

常见白光偶联物有：Biotin生物素、HRP辣根过氧化物酶、AKP碱性磷酸酶。

 

常见荧光偶联物有：Fitc、Cy3、Cy5、AF488、AF555、AF647；

注意现在常用的偶联物是偶联在二抗上的，但是一抗的选择决定了能不能用于某种偶联物的

应用种属

 

接着根据你的实验样本来选择一抗，通常一抗的适用种属都有三种Mouse、Rat、Human，但有的适用于其中一种或两种，看清楚了，这将决定你的实验的成败。

 

来源种属

 

通常一抗的来源种属有Rabbit和Mouse，二抗的来源种属有山羊和驴。

 

这里要注意的是避免种属间的交叉反应造成的假阳性，比如样本是小鼠的，一抗尽量用兔抗，不要用鼠抗，不然山羊抗小鼠二抗有可能会和样本中的某些蛋白发生非特异性反应，进而造成假阳性。

克隆号、表位

筛选到这里已经能排除掉很大一部分抗体了，如果不是有特殊的需求，到这里就可以从筛结果里随意的选择了。

大鼠组织蛋白酶K(CTSK)ELISA试剂盒 Rat CTSK (Cathepsin K) ELISA Kit

大鼠组织蛋白酶L(CTSL)ELISA试剂盒 Rat CTSL (Cathepsin L) ELISA Kit

大鼠组织多肽抗原(TPA)ELISA试剂盒 Rat TPA (Tissue Polypeptide Antigen) ELISA Kit

大鼠组织纤溶酶原激活物(tPA)ELISA试剂盒 Rat tPA (Plasminogen Activator, Tissue) ELISA Kit

大鼠组织因子(TF)ELISA试剂盒 Rat TF (Tissue Factor) ELISA Kit

大鼠组织因子途径抑制物(TFPI)ELISA试剂盒 Rat TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit

大鼠组织转谷氨酰胺酶(TGM2)ELISA试剂盒 Rat TGM2 (Transglutaminase 2, Tissue) ELISA Kit

大鼠胰岛素自身抗体（IAA）ELISA试剂盒 Rat IAA (Insulin Autoantibodies) ELISA Kit

大鼠利钠肽受体1(NPR1)ELISA试剂盒 Rat(NPR1)ELISA Kit

大鼠利钠肽受体1(NPR2)ELISA试剂盒 Rat(NPR2)ELISA Kit

大鼠利钠肽受体1(NPR3)ELISA试剂盒 Rat(NPR3 )ELISA Kit

大鼠细胞色素P450 3A4（CYP3A4）ELISA试剂盒 Rat(CYP3A4 )ELISA Kit

大鼠I型胶原氨基端肽 CTX-1）ELISA试剂盒 Rat（CTX-1）ELISA Kit

大鼠内源性糖皮质激素（GC）ELISA试剂盒 Rat（GC）ELISA Kit

大鼠丝氨酸棕榈酰转移酶长链碱性亚基2(SPTLC2)ELISA试剂盒 Rat（SPTLC2）ELISA Kit

大鼠丝氨酸棕榈酰转移酶长链碱性亚基1(SPTLC1)ELISA试剂盒 Rat（SPTLC1）ELISA Kit

大鼠丝氨酸棕榈酰转移酶长链碱性亚基3(SPTLC3)ELISA试剂盒 Rat（SPTLC3）ELISA Kit

大鼠三磷酸肌醇(IP3)ELISA试剂盒 Rat（IP3）ELISA Kit

大鼠雌激素（E）ELISA试剂盒 Rat（E）ELISA Kit

大鼠Ⅰ型胶原C端肽(CTX-Ⅰ)ELISA试剂盒 Rat（CTX-Ⅰ）ELISA Kit

大鼠类胰蛋白酶（TPS）ELISA试剂盒（活性）

大鼠髓过氧化物酶(MPO)ELISA试剂盒 Rat（TPS）ELISA Kit